Flow cytometry of ECE1 in MCF-7 human breast cancer cell line. Samples were incubated in ECE1 monoclonal antibody (Product # MA5-24016) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab)2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Flow cytometric analysis of MCF-7 human breast cancer cell line was stained with Rat Anti-human ECE-1 Monoclonal Antibody (Product # MA5-24016) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F (ab')2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Supplier Page from Thermo Fisher Scientific for ECE1 Antibody